Human Chromosome Karyotyping and Molecular Biology by Flow Cytometry

摘要

Flow cytometry is a sensitive analytical tool for rapdily measuring the biological, chemical and physical properties of cells and cellular components, such as chromosomes and has become a promising system for automating human chromosomal karyotyping. Unlike traditional approaches based upon chromosomal length, centromeric index, and banding patterns, it is based on the measurement of chromosomal DNA content and base composition and can classify chromosomes more objectively. In this paper we describe flow karyotyping and chromosome sorting and compare flow cytometry with the use of cell hybrids for gene mapping and the construction of chromosome-specific genomic libraries.