Inhibition of Baboon Marrow CFU-GEMM, CFU-GM, BFU-E and CFU-E by Adrenochrome, an Epinephrine Metabolite

摘要

Using tissue culture systems designed for the growth of human bone marrow cells, studies were done to assess the effects of adrenochrome, a metabolite of epinephrine, and of hemin on baboon bone marrow CFU-GEMM, CFU-GM, CFU-E and BFU-E. In all the assay systems studied, growth of CFU-GEMM, CFU-GM, CFU-E and BFU-E was suppressed by the addition of 0.5 mM adrenochrome. For those clonal cell assays expressing erythroid growth in culture, the degree of suppression by 0.5 mM adrenochrome increased with increasing cellular maturity. The addition of 0.2 mM hemin alone produced a slight but not statistically significant depression of baboon marrow BFU-E growth. The combination of hemin and 0.5 mM adrenochrome produced no growth of BFU-E, but did produce a significant increase in the CFU-GEMM as compared to 0.5 mM adrenochrome alone. These data suggest that substances such as adrenochrome may be involved in the suppression of red cell production observed in patients subjected to traumatic injuries.