Sister Chromatid Exchange, DNA Repair, and Single-Gene Mutation

摘要

Sister chromatid exchange (SCE) has been studied in cultured mammalian cells with regard to the nature of the inducing lesion, mutation induction, and factors that modify the observed frequency following mutagen exposure. SCEs can be induced by a wide spectrum of DNA lesions and, for nine agents examined, the frequency of induced SCE is linearly related to induced single-gene mutation. Further, a deficiency in DNA repair may alter the expression of both SCE and mutation in a qualitatively similar manner. The frequency of SCE induced by mitomycin-C is suppressed in heterochromatin relative to euchromatin and, in non-dividing lymphocytes, the lesions leading to the formation of SCEs may persist for several months. (ERA citation 06:019265)