Ultramicro Methods in Biochemistry. VII. The Determination of Plasma or Serum Urea Nitrogen. VIII. The Determination of Serum Uric Acid. IV. The Determination of Serum Creatinine

摘要

Ultramicro methods for the determination of serum urea nitrogen, uric acid, and creatinine have been evaluated and the results are recorded in reports VII, VIII, and IX, respectively. The method for the determination of urea nitrogen is an ultramicro adaptation of the reaction in which a blue color is developed when the ammoniumion reacts with phenol and hypochlorite. Ammonia is released from urea with urease. The entire reaction is conducted on 5micronl of serum or plasma and the final volume of reaction mixture is 2. 205 ml.