BONE MINERALIZATION IN TISSUE CULTURE:THE CALCIUM-PK0SPHATE PRODUCT AND RATIO

摘要

Using a tissue culture system, the mineralization of both whole and longitudinally split chick embryo tibias was shown to be directly related to the medium Ca/PO4 ratio when the Ca X PO4 product was constant. However, with increasing medium Ca levels, mineralization of the split bones was much more extensive than that of the intact bones. Lactate production by the split tibias was independent of the medium Ca/PO4 ratio and was less than the Ca/PO4 ratio-dependent production by the intact bones. All of the cultured bones showed an increase in total nitrogen that was independent of either splitting or medium Ca/P04 ratios. The results of this study support the recent concept of a metabolically active bone membrane