Detection of N-Acetylglycine, N-Acetylglycylglycine,and N-Acetylglycylglycylglycine by Paper Chromatography.

摘要

This paper reports that the developing solvent of Kirchenmayer and Kuffner, n-butanol-acetic acid-water (8:1:10, v/v) can be used to separate and detect N-acetylglycine, N-acetylglycylglycine,and N-acetylglycylglycylglycine. The chromatographic procedure is described. After heating at 100exp 0 C,the spots could be seen under uv light;acetone-bromocresol green-morpholine can also be used to make the spots show up. The R/sub f/values are 0.59, 0.43,and 0.33,respectively. This procedure was used to detect these compounds in aqueous solutions of diglycine, triglycine,and tetraglycine,which were used as gamma-radiolysis targets. (ERA citation 03:050792)