Detection and Characterization of Novel Metal-Binding Proteins. First Year Report, 1984-1985

摘要

We have synthesized several model peptides based on the metallothionein (MT) protein sequence, and have used these, in conjunction with several naturally occurring proteins, to evaluate current methodologies for isolating and characterizing metal-binding proteins. Our off-contract research has indicated that High Pressure Liquid chromatography (HPLC) offers several distinct advantages compared to other techniques in this area. In addition, we have developed improved on-line analysis for the detection of sulfhydryl-containing proteins that have been separated by HPLC. 12 figs., 2 tabs. (ERA citation 11:007844)