Coupling of Glucose Oxidase and Potassium Ferrocyanide as a Basis for a Simple Colorimetric Estimation of Glucose

摘要

The coupling of glucose oxidase and Fenton's reagent for a simple, inexpensive assay of glucose has been described. In the reaction, hydrogen peroxide, formed as a result of the action of glucose oxidase on glucose, oxidizes ferrous to ferric sulfate which results in an increase in the absorbance of ultraviolet light at 340 nm. It was proposed that the assay would be useful to measure the activity of enzymes such as cellulase and beta -glucosidase. Potassium ferrocyanide (colorless) when oxidized to potassium ferricyanide results in a yellow color with an absorbance maximum at 420 nm. Described here are some preliminary results indicating that the interaction between hydrogen peroxide and potassium ferrocyanide results in an increase in absorbance which is maximal at 420 nm. A simple assay for glucose based upon this interaction might be useful under some circumstances. 3 refs., 4 figs. (ERA citation 11:028411)