Simple and Efficient Production of Hybrid Oligomeric Enzymes by Allelic Complementation: Subunit Interactions of Ribulose Bisphosphate Carboxylase/Oxygenase.

摘要

The in vitro production of hybrid enzyme has been used to examine subunit interactions and the formation of shared active sites. Recently, we described a functional test of a shared active site of ribulose bisphosphate carboxylase/oxygenase by coexpression of engineered mutants. Unlike in vitro complementation methods, which require reversible dissociation and reassociation of proteins, in vivo allelic complementation utilizes physiological assembly processes. Here, we describe the use of compatible expression plasmids which should be of general utility for the production of hybrid enzymes. 8 refs., 1 fig. (ERA citation 13:041396)