Recombinant DNA Method for Understanding the Mechanism of Salmonella Reverse Mutations: Role of Repair.

摘要

This is a report of an investigation of the specific changes in the DNA of Salmonella revertants induced by environmental mutagens and their metabolites. These compounds were chosen because they specifically induce frameshift lesions in strains TA1538 and TA98. DNA lesions in these strains are analyzed in the hisD gene and can lead to reversion of histidine dependence by either deletions, insertions, or suppressor mutations. The understanding of the types of DNA-base changes induced by both a mutagen and its metabolites can lead to a better understanding of not only the mutational process and repair mechanisms, but also the potency and mode of action of specific metabolites and their corresponding DNA adduct(s). The detailed development of the methods used for this research and the original findings with benzo(a)pyrene have been recently published. 4 refs., 2 figs. (ERA citation 14:003589)