Preparation of Iodine-125 - Labeled Insulin for Radioimmunoassay: Comparison of Chloramine T and Iodogen Iodination.

摘要

Stoichiometric iodination of porcine insulin was performed to the general method of Hunter and Greenwood with modifications recommended by Roth. These method was compared with radioidination using Iodogen. Films of Iodogen react rapidly in the solid phase with aqueous mixtures of I (sup -)and proteins. For two methods satisfactory activity of the labeled porcine insulin was obtained and characteristics of the radioimmunoassay were studied. (Atomindex citation 19:088287)