DOE project on genome mapping and sequencing. Progress report, 1992

摘要

These efforts on the human genome project were initiated in September, 1990, to contribute towards completion of the human genome project physical mapping effort. In the original application, the authors proposed a novel strategy for constructing a physical map of human chromosome 11, based upon techniques derived in this group and by others. The original goals were to (1) produce a set of cosmid reference clones mapped to specific sites by high resolution fluorescence in situ hybridization, (2) produce a set of associated STS sequences and PCR primers for each site, (3) isolate YAC clones corresponding to each STS and, (4) construct YAC contigs such that > 90% of the chromosome would be covered by contigs of 2 mb or greater. Since that time, and with the advent of new technology and reagents, the strategy has been modified slightly but still retains the same goals as originally proposed. The authors have added a project to produce chromosome 11-specific cDNAs and determine the map location and DNA sequence of a selected portion of them.