Evaluation of a Sandwich Gene Probe Assay for Newcastle Disease Virus

摘要

A sandwich gene probe assay was evaluated in comparison to a direct gene probeassay. The target sequence used in each of the assays was a 673 bp DNA fragment of the major nucleocapsid protein gene of NDV. In the direct probe assay, the 673 bp DNA fragment was labelled with digoxigenin and hybridized to unlabelled 673 bp target DNA. In the sandwich assay, the target DNA was detected using two probes. The primary probe was unlabelled, recombinant M13mp18 viral DNA containing the 673 bp gene fragment which hybridized to the 673 bp target DNA. The secondary