Comparative Genomic Hybridization Onto Dense Arrays of DNA Clones: Development and Application to Breast Cancer Genomes

摘要

The work under the final portion of U.S. Army grant DAMD17-96-1-6165 focused on the technical aspects to produce high density arrays in automated fashion. A printing robot was constructed that used a capillary print head developed in the first year of this grant. This printer was shown to produce arrays that performed as well as our previous hand-spotted arrays. Much less target DNA is necessary, permitting faster, cheaper automated clone purification procedures, such as the Autogen machine. Finally, I developed an improved narrow bore capillary print head capable of printing arrays of densities 104/cm2.