Retrotransposons as Mutagens in Human Breast Cancer. Addendume

摘要

It is known that the human LINE-1 retrotransposon (L1Hs) is transcriptionally active in many breast cancer cells. The original goal of the project was to test the possibility that L1Hs retrotransposition events result in the inactivation of tumor suppressor genes by insertional mutagenesis. A tagged L1Hs element was to be stably integrated into a nonmalignant breast cell line (MCF10A) and allowed to retrotranspose. Malignant cells would then be identified and the genes into which the L1Hs element had integrated could be isolated using the tag.