Rational Derivatives of Maspin as Candidate Tumor Suppressive Agents of Breast Cancer

摘要

We proposed to generate and characterize rational derivatives of maspin as candidate tumor suppressive agents of breast cancer. We plan to produce these novel maspin variants as purified proteins (Specific Objective 1), and as re-expressed proteins in breast tumor cells that are transfected with the corresponding coding cDNAs (Specific Objective 2). The biological functions of these maspin derivatives as well as the wild type maspin will be examined using comprehensive cellular and biochemical assays that have been established in my laboratory. In particular, we planed to focus on the effect of these molecules on tumor growth, cell adhesion, cell motility and invasion. In addition the biochemical and biophysical properties of these new molecules will be evaluated.