DURIP: Super-Resolution Module for Confocal Microscopy of Reconfigurable Matter.

摘要

This project acquired an instrument module for super-resolution confocal microscopy imaging of reconfigurable colloids. During the project the different methods and instruments available for super-resolution microscopy was evaluated against the needs of colloidal assembly and soft matter science. The techniques and instruments evaluated were stimulated emission depletion microscopy (STED), stimulated illumination microscopy (SIM), and photoactivated localization microscopy (PALM). Our evaluation determined that the SIM method is currently the super-resolution method most compatible with our group s needs in soft-matter assembly. Furthermore, demonstration testing established that the performance of commercially available SIM modules were significantly better than the currently best available resolution of confocal microscopy.