Molecular Markers of Metastasis in Ductal Mammary Carcinoma

摘要

Representational difference analysis method was used to isolate metastasis-specific genetic markers for breast cancers that are prone to develop metastasis. Of the 15 candidate MADS that have been isolated, 3 were found to be promising markers for breast metastasis. Screening of MADS-IX on a tumor cell line with homozygous loss of PTEN gene, revealed that MADS-IX is neither a part of PTEN gene nor localized in the homozygous loss region of chromosome 10q arm encompassing PTEN. Our Fluorescence in situ hybridization screening for MADS%S on primary tumor tissue touch preparation cells was successful. The results indicate that the MADS-IX could be used as a FISH probe for distinguishing the primary tumors that did and that did not develop metastasis.