Real-Time PCR Diagnostics for Detecting and Identifying Potential Bioweapons

摘要

Partial contents: Diagnostics Systems Division, Diagnostics and Patient Care: Goals, Diagnostic Essentials, Impact of Diagnostics and Patient Care, Diagnostic Time Constraint, Classical Methods for Identifying Biological Agents, Diagnostic Sensitivity Requirements, Integrated Identification and Diagnostic System, Real-Time PCR, Quantitative PCR, Sample Types, Biomarkers, Determine Gene Target(s), Taqman Chemistry, Common Real-Time PCR Chemistry: TaqMan-MGB, Primer Design Guidelines, Probe Design Guidelines, Taqman MGB Probes, Taqman MGB Probes for Allelic Discrimination, Design Software, Taqman Optimization, Chemistry, Typical Cycling Parameters, Sensitivity/Optimized Assay.