Development of COMET-FISH Method for Rapid Identification of Persons at Risk for Breast Cancer Development. Addendum

摘要

A proportion of breast cancers have genetic alterations at 17q11.2- q12 (HER-2/neu), 20q13.2 (ZNF217 gene) and 17p13.1 (p53). We have documented global genetic instability within malignant breast cancer cell lines (MCF-7; MDA-MB-468 and CRL-2336) by coupling the DNA-damage Comet assay to fluorescence in situ hybridization (Comet-FISH). Gene-specific instability at p53 and HER-2 loci was also observed. Following genotoxic insult (IR and H2O2), we found that p53 locus was capable of rapid and preferential repair when compared to the HER- 2 locus. Comet-FISH is a rapid and easy assay which is amenable for the study of gene- specific instability or DNA repair in tissues relating to cancer risk or progression.