Proteomic Analysis of Bronchoalveolar Lavage Fluid: Effect of Acute Exposure to Diesel Exhaust Particles in Rats

摘要

Inhalation of diesel exhaust particles (DEP) is characterized by lung injury and inflammation with significant increases in the numbers of polymorphonuclear leukocytes and alveolar macrophages. This influx of cellular infiltrates is associated with the activation of multiple genes, including cytokines and chemokines, and the production of reactive oxygen species. The pathogenesis of the lung injury is not fully understood, but alterations in the presence or abundance of a number of proteins in the lung have been observed. The present study investigates global alterations in the proteome of bronchoalveolar lavage fluid taken from rats 1, 7 or 30 days after exposure to 5, 35, or 50 mg/kg of animal weight of DEP. Analysis by surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF) identified two distinct peaks that appeared as an acute response post-exposure at all doses in all animals. These two peaks, with mass to charge ratios (m/z) of 9, 000 and 10,100 were identified as anaphylatoxin C3a and calgranulin A by additional mass spectral investigation using liquid chromatography coupled to mass spectrometry (LC/MS).