Production of 2-Amino-5-Phenoxyphenol from 4-Nitrobiphenyl Ether Using Nitorbenzene Nitroreductase and Hydroxylaminobenzene Mutase from Pseudomonas Pseudoalcaligenes JS45 (Postprint).

摘要

Microbial metabolism of nitroarenes via 0-aminophenols requires the participation of two key enzymes, a nitroeducatase and an hydroxylaminobenzene mutase. The broad substrate ranges of the enzymes suggested that they could be used as biocatalysts for the production of substituted 0-aminophenols. We have used enzymes from Pseudomonas pseudoalcaligens JS45 for the conversion of 4-nitrobiphenyl ether to the corresponding 0-aminophenol. Partially purified nitrobenzene nitroeductase reduced 4-nitrophenyl ether to the corresponding 4-hydroxylaminobiphenyl ether. Partially purified hydroxylaminobenzene mutase stoichiometrically converted the intermediate to 2- amino-5-phenoxyphenol. The results indicate that the enzyme system can be applied for the production of 0-aminophenols useful as intermediate for synthesis of commercially important materials.