Structural Studies on Toxins and Virulence Factors of Yersinia pestis

摘要

In this final report we present our efforts on two or three fronts. We were working on the expression purification and crystallization of complexes of YopB and YopD with other proteins like their chaperones. We have made progress in purifying YopB:YopD:SycD complex. However this is a challenging process and the amount of homologous protein is a problem. Since the expression levels of different components of the complex are different the stoichiometric ratio is a problem. We will continue to work on this project to successfully end it even though the project period has ended. If successful this will help in understanding the translocation mechanism of effector proteins.