Deposition of Selected Airborne Particles into a Microfluidic Flow Cytometer for Bioanalysis; Final rept

摘要

In a proof-of-concept experiment, we demonstrate how selected airborne bacteria, detected in air by light scattering, can be deflected aerodynamically into the input well of a microfluidic flow cytometer and analyzed using fluorescein-labeled antibodies. This experiment demonstrates a new method for introducing airborne samples into a microfluidic cell. The results suggest that by deflecting only those airborne particles having the fluorescence spectra of bioaerosols and by depositing the individual bioaerosols into the smallest volume of reagent required for analysis, it should be possible to achieve a system that can continuously monitor the air for certain bioaerosols while minimizing reagent usage.