Novel Combination of Thermal Ablation and Heat-Inducible Gene therapy for Breast Cancer Treatment

摘要

High intensity focused ultrasound (HIFU) has been developed as an emerging non-invasive strategy for cancer treatment by thermal ablation of tumor tissue. The feasibility of synergistic combination of HIFU thermal ablation and HIFU-induced gene therapy is interpretedboth in vitro and in vivo using cancer cell lines (HeLa, 4T1 and R3230Ac) transfected with mark gene encoding proteins (GFP and Luciferase)under the control of Hsp70B promoter. Moreover, a transparent cell-embedded hydrogel is developed to allow for simultaneous assessment of lesion formation, gene expression and temperature distribution. This work opens up a new paradigm for synergistic combination of HIFU thermal ablation with heat-induced gene therapy to improve the overall quality and efficacy of breast cancer therapy.