Regulation of the Inflammasome, a Modulator of Caspase-1 Mediated Cytokine Production

摘要

Aberrant regulation of the epidermal growth factor receptor is implicated in a number of epithelial cancers, including a large fraction of breast cancers. Antibody based inhibitors directed against the extracellular ligand binding domain of the receptor have proven to be a clinically viable cancer therapeutic. We have used mutagenesis and binding competition studies to map the epitopes of two inhibitory antibodies. Additionally, we have structurally and biochemically characterized a set of inhibitory single-domain VHH antibody fragments directed against the exctracellular domain of the receptor.