Determining Insulin Levels Using Insulin I 125 and Insulin-Binding Reagent

摘要

A procedure for the immunoassay of insulin levels using insulin I 125 as a tracer was evaluated to study parameters of reproducibility, accuracy of values, sensitivity with changes in fasting times, temperature effects, and recovery. On the basis of the evaluation, the method is considered to be appropriate as a routine diagnostic test for fasting insulin levels. The usefulness of this procedure for measurement of insulin levels through the course of a standard glucose test has yet to be evaluated. Future study should involve evaluation of the concept of free and bound insulin, determining which insulin this system measures, and the relationship of free to bound insulin during both fasting and glucose tolerance conditions in normal individuals. (Author)