FATIGUE AND STRESS STUDIES: AN IMPROVED SEMI-AUTOMATED PROCEDURE FOR FLUOROMETRIC DETERMINATION OF PLASMA CATECHOLAMINES,

摘要

A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization,and fluorescence detection are accomplished by an automated chemical system. Recoveries from plasma of 0.01and 0.02micrograms total catecholamines/10-ml sample were 86.4% plus or minus 8.9% (SD) and 85.6% plus or minus 5.8% (SD),respectively. Total catecholamine level in resting human plasma,determined by the present technique,was 0.89plus or minus 0.15 (SD) micrograms/l. (Author)