Zinc Sulfate Failure as Accelerator of Collagen Biosynthesis and Fibroblast Proliferation

摘要

The effect of zinc sulfate on collagen biosynthesis and fibroblast proliferation has been studied in a tissue culture model system using human skin fibroblasts. Addition of zinc sulfate to newly established (low density) cultures in concentrations of 0.0001 to 10 to the -8th power M results in concentration-dependent cytotoxicity with inhibition of collagen biosynthesis as well as cellular proliferation. Zinc sulfate at 0.0001 to 0.0000001 M added to confluent (high density) cultures does not influence cellular proliferation but results in selective inhibition of collagen biosynthesis at the 0.0001 M concentration. Lower concentrations of zinc sulfate do not affect collagen biosynthesis in high density cultures. The results show that zinc does not have a direct acceleratory effect on cellular proliferation or collagen biosynthesis in human skin fibroblasts in tissue culture. They also suggest that the acceleratory effect of zinc in wound healing may not involve these parameters. (Author)