Stimulation by Aldosterone of the Sodium Efflux in Barnacle Muscle Fibres: Effects of RNA Inhibitors and Spironolatone.

摘要

Single muscle fibres from the barnacle Balanus nubilus have been studied to provide information about the mode of action of aldosterone on Na transport in symmetric cell. Neither internal nor external application of aldosterone modified the course of the Na efflux. Internal application of aldosterone in a concentration as low as 10 to the minus 10M power promptly stopped the rate constant for Na efflux from further falling but failed to cause delayed stimulation. Internal application of actinomycin-D, alpha-amantin or cordycepin shortly before application of external aldosterone caused complete abolition of the delayed stimulation. Internal application of spironolactone SC-14266 shortly before external application of aldosterone caused complete abolition of the biphasic response to the steroid.