Studies of Dogs Subjected to Acute Blood Loss and Transfused with Blood Stored in ACD with and without Adenine.

摘要

Transfusion of stored blood after acute hemorrhage in a canine experimental model was observed. The transfused blood had been stored in acid-citrate-dextrose with or without adenine for 14, 28,or 42days. Diphosphoglycerate,adenosine triphosphate,and pH levels decreased with storage,while the oxygen curve shift to the left was indicated by the decrease in P50. After transfusion,low values of diphosphoglycerate and P50were observed in the recipient,but these usually returned to normal by 24hr. The depression of values and recipient response were similar with or without adenine. During the hemorrhage and hypovolemic period, P50and diphosphoglycerate values were found to be higher than normal. A normal pH was observed during bleeding,but it fell during hypovolemia. Because the bleeding was accomplished in 15min,the elevated P50and diphosphoglycerate values would indicate a rapid response of the oxygen transport system. (Author)