Ultrastructural Pathogenesis of Lesions Produced by Exposure to Oxygen Difluoride with Correlative Light Microscopy,

摘要

The lungs of rats exposed to OF2were examined by light and electron microscopy. The exposures were for 30to 60minutes to an average of 4.5ppm OF2,the minimal lethal dose. Animals were sacrificed after 30 (group 1) and 60minutes (group 2) exposure and 1 (group 3) and 2 (group 4) hours following 60minutes exposure. Mild gross changes were observed in groups 3and 4,but no light microscopic lesions were found. Alterations were noted in all four groups using electron microscopy. These were mostly indicative of fluid change and consisted of blebbing of the endothelial and epithelial layers of the alveolocapillary wall and rarification of the cytoplasm of these cells. The lamellar bodies of the Type II cells showed an increasing and consistent loss of matrix structure and density. These fine structural changes increased in quantity and severity as time of exposure or post-exposure period increased. (Modified author abstract)