Separation of Lymphocyte Mitogen from Lymphotoxin and Experiments on the Production of Lymphotoxin by Lymphoid Cells Stimulated with the Partially Purified Mitogen: A Possible Amplification Mechanism of Cellular Immunity and Allergy.

摘要

A mitogenic factor (MF) from guinea pig lymph node cells (LNC), which was produced by stimulating immune LNC with the specific antigen, ovalbumin, was partially purified by the sequential use of Sephadex G-200 gel filtration, CM-cellulose ion exchange chromatography, DEAE-cellulose ion exchange chromatography, and polyacrylamide disc gel electrophoresis. The possibility that MF, or a substance closely assoicated with this factor, might serve to amplify cellular immune and allergic reactions by nonspecifically inducing lymphoid cells to produce other lymphokines was then tested by stimulating cells from various lymphoid tissues with the purified MF and assaying the culture fluids for LT activity as a measure of lymphokine production. Purified MF, or a substance closely associated with this factor is capable of inducing LT production by a subset of lymphoid cells that appears to be expanded after immunization. Electrophoretic observations indicated that the induction of LT production may be due to a factor which is distinct from MF although closely similar in molecular size and charge.