Removal of HB(s)Ag from Blood In Vitro. I. Effects of Washing Alone, Glycerol Addition and Removal, and Glycerolization, Freezing, and Washing.

摘要

Red blood cells from HB(s)Ag-positive blood were washed in the Fenwal Elutramatic, Haemonetics Processor 15, or the IBM Blood Processor with sodium chloride solutions, or in the Huggins Cytoglomerator with sugar solutions. The Fenwal Elutramatic and IBM Blood Processor were the most efficient washing systems, the Haemonetics Processor 15 was less efficient, and the Huggins Cytoglomerator was the least efficient in removing the HB(s)Ag. Washing to remove the HB(s)Ag from red blood cells containing 40 per cent W/V glycerol in an ionic medium was more efficient than washing HB(s)Ag from liquid-stored red blood cells or red blood cells containing 20 per cent W/V glycerol. The original and modified dilution/agglomeration wash cycles used in the Huggins Cytoglomerator were not able to remove the HB(s)Ag from units of blood that were radioimmune assay (RIA) positive and counterelectrophoresis (CEP) negative. Freezing had no effect on the removal of the HB(s)Ag in vitro, whereas the concentration of 40 per cent W/V glycerol in the red blood cells that were washed did.