Pathophysiological Responses of the Rhesus Monkey to Live 'Escherichia coli'.

摘要

The present study was designed to develop an animal model applicable to the clinical patient in the investigation of the pathogenesis of septic shock. The currently described model is a lightly anesthetized, unrestrained monkey, carefully monitored during a twenty-four hour observation period. Varying doses of live E. coli organisms were intravenously infused during a thirty minute period, and a variety of hemodynamic, respiratory and metabolic parameters were monitored. Two controls, not administered organisms, survived the twenty-four period with minimal changes in all measured parameters. Results reveal two patterns in response to organism administration:early acute death (3-4 hours) and prolonged life (death between 20-27 hours). The acute response was characterized by marked systemic hypotension, hypoglycemia, hypoinsulinemia, increased lactate, decreased pH or respiratory depression. The other type of response involved profound sustained hypotension with hypoglycemia and hypoinsulinemia in most but not all animals, and elevations in lactate, BUN, potassium, creatinine, SGOT, LDH AND F-LDH. Depressions in respiration were not evident in the longer surviving group. Renal fibrin thrombi, porminent in baboons administered E. coli, were absent in the rhesus monkey regardless of dosage of arganisms employed. This study suggests the operation of a multifactorial mechanism of septic shock with interactions between hemodynamic and metabolic factors varying within the species.