Preparation of I-125 Polypeptide Hormones for Radioimmunoassay Using Glucose Oxidase with Lactoperoxidase.

摘要

The hydrogen peroxide-generating property of glucose oxidase was used in combination with the catalytic property of lactoperoxidase to radioiodinate polypeptide hormones with 125-iodine. This rapid, gentle, enzymatic method reproducibly results in higher yields of iodinated hormones for use in radioimmunoassays. (Author)