Common Blood Bank Contaminants Evaluated by the Bactec Radiometric System.

摘要

Research techniques in blood preservation require frequent entry into blood bags stored at 4 C. Rapid detection of possible contamination and careful evaluation of a selected bacteriological method are essential. Bactec(Registered) radiometric methods (Johnston Laboratories, Inc., Cockeysville, MD) were evaluated. Data were collected concerning detection of small numbers of organisms (1 to 1000 colony forming units per milliliter). Sudies were done with 0.5 to 3 ml/vial inoculum and at temperatures of 35, 22-24, and 4 C. The evidence indicates that the Bactec radiometric procedure is adaptable to smaller samples and to different incubation temperatures than recommended by Bactec for non-fastidious organisms only. Oxidative psychrophilic and other fastidious organisms were detected more frequently and faster using subculture and gram stain methods. (Author)