Improved Method for Measuring Concentrations of Carnitine and Its Derivatives in Tissue Extracts.

摘要

A method was developed to measure carnitine and short-chain and long-chain acylcarnitines in perchloric acid extracts of liver and muscle. The radioisotopic assay employing (1-C(14)acetyl-CoA and the measurement of labeled acetylcarnitine resulted in a sensitivity range of < 1 to 50 nmol L-carnitine/50-microliter sample. Samples frozen at -20 C revealed no losses in carnitine concentrations for several months. Recovery of the carnitine added to perchloric acid extracts of liver was 103 + or - 2%. (Author)