Effect of Topically Applied Diisopropylfluorophosphate on Glucose Metabolism in the Rat

摘要

A solution of DFP in peanut oil was applied topically on the clipped mid-Lumbar region of rats and two hours later alterations in glucose utilization by skin, liver and adipose tissue preparations were determined. DFP had no demonstratable effect on glucose oxidation. In contrast, DFP enhanced fatty acid synthesis by 70% over the control values in the skin and by 56 and 92% in the liver and adipose tissue, respectively. DEP stimulated synthesis of cholesterol and sterols by 69% in the liver and doubled and tripled the synthesis, respectively, in the skin and adipose tissue. DFP effects on glycogen synthesis appear to be the result of a decreased activity of the key enzymes in the synthetic pathway. Enhanced synthesis of various lipid components appears to be substrate-induced.