Toxicity of Perfluoro-N-Decanoic Acid and 2,3,7,8-Tetrachlorodibenzo-P-Dioxin in L5178Y Mouse Lymphoma Cells

摘要

Perfluoro-n-decanoic acid (PFDA) causes toxic sequelae in vivo very similar to those caused by 2,3,7,8-tetrachlorodiobenzo-p-dioxin (TCDD). The toxicity of these two compounds, several other polyfluorinated fatty acids, and corresponding hydrogenated fatty acids have een studied in vitro in L5178Y mouse lymphoma cells. Below concentrations which cause cell lysis (>or=500 micro g/m1), PFDA did not affect suspension growth. After 24 hr treatment with concentraions between 1 and 100 micro g/m1 treated cells no longer grew into clones when plated in semi-soft agar. This impairment of clone-forming ability was reversible after growth of treated cells in fresh medium for 36 hr. Perfluoro-n-octanoic acid did not impair clone-forming ability at any concentration; and neither did the straight-chain hydrogenated fatty acid analogs. All polyfluorinated acids tested (either perfluorinated or w-hydro-analogs) with chain length 9 or greater caused impairment of clone-forming ability after treatment with concentrations that were non-toxic in suspension. TCDD (highest dose, 0.5 micro g/m1) had no effect on growth in suspension. In vivo PFDA treatment altered erythrocyte fragility in rats. It is suggested that the toxicity of PFDA and TCDD in vivo and in L5178Y cells in vitro may be due to an ability of these chemicals to interfere with normal structure and/or function of biological membranes.