Effects on Electromagnetic Fields on Calcium Effleux from Monolayer Cultures of Brain Cells Isolated from Embryonic Chick Cerebral Hemispheres

摘要

The results presented herein demonstrated that microwave irradiation of alert rats at 2450 MHz can cause an alteration in the uptake of 2-deoxyglucose in the frontal and dorsal portions of the CNS. This increase could result from a) a direct heating of these CNS regions with a resultant increase in the brain's demand for glucose, b) direct local heating with a resultant increase in neuronal activity which in turn increases demand for glucose, or c) an indirect effect of glucose on some other portion of the body with an increased neuronal input to the affected brain regions which in turn increase their electrical activity and incorporate more 2-deoxyglucose. A technique recently developed in this laboratory has been used to determine whether sinusoidally modulated (0, 9, or 16 Hz) microwave irradiation (41.8 GHz, 0-2 mW/sq cm) can alter calcium efflux from brain tissues. Monolayer cultures of neurons and glia were prepared from the cerebral hemispheres of 9-10 day chick embryos and the cells were prelabelled with 45 Ca. All culture dishes were placed directly on the open end of waveguide at 37.2 C and were either sham irradiated or irradiated for 20 min. It was possible to assess the effect of the modulated weak microwaves at 24 power densities ranging from zero at each edge to 2 mW/sq cm at the center of the waveguide. An analysis of calcium binding by microwave- and sham-irradiated cultures demonstrated that irradiation had no effect on calcium binding at any power density up to 2 mW/sq cm and at any of the three modulating frequencies.