Notes on Use of the Single Solution Ascorbic Acid Method for Phosphate Determinations: An Amended Method

摘要

The project objectives were to define the sources of potential inaccuracy and imprecision in ortho-phosphate analyses and to find a practical and reliable solution to quantitation. A modification to the current 'standard method'; involves heating samples, after addition of the color developer reagent, at 75 C for ca. 20 minutes. The modification yields quantitative results with high precision, high accuracy and birtually no constraint on the timing of absorbance measurements.