Choline Uptake by Glomerular Synapses Isolated from Bovine Cerebellar Vermis

摘要

Tritium Choline uptake was investigated in a highly enriched preparation of glomerular particles isolated from bovie cerebellar vermis. Kinetic analysis indicates that a high affinity uptake sytstem with a relatively low maximum uptake velocity is present. At a substrate concentration of .0000001 M, Tritium choline uptake was shown to be sodium-dependent, hemicholinium-sensitive and nonreactive to increasing concentrations of unlabeled choline. These findings support the presence of a high affinity choline uptake system in cerebellar glomeruli and would seem to be consistent with the suggestion that a small proportion of mossy fibers are cholinergic in this brain region.