Multi-Enzyme Complexes in the Thermophilic Archaea: The Effects of Temperature on Stability, Catalysis and Enzyme Interactions in a Multi- Component System.

摘要

Determine the thermostability and thermoactivity of the individual complex components. Study the temperature dependence of whole complex assembly using analytical ultracentrifugation and laser light intensity fluctuation spectroscopy. Investigate the post-translational modification of the E2 component; that is, the lipoylation of the specific lysine in the lipoyl domain. This involves cloning of the genes for the relevant lipoylation enzymes, and characterisation of the protein products.