Production of Rh sub 0 (D) Negative Erythrocytes

摘要

Attempts are being made to modify or destroy the Rh D antigen under conditions which will produce Rh negative erythrocytes of transfusable quality. One of the approaches being taken involves the isolation and biochemical characterization of the D antigen in order to gather information which will help formulate ways of modifying its expression. To this end we have progressed toward establishing a 28-32 Kd membrane protein as the primary D antigen. This is evidenced by its ability under certain conditions to induce antibody toward Rh positive cells and when attached to plates to yield a positive reaction with human anti-D in the ELISA assay.