HLA-Dw and HLA-DP Typing of the Reference Panel of B-Lymphoblastoid Cell Lines.

摘要

Cellular typing for HLA-Dw and HLA-DPw within the Tenth International Histocompatibility Workshop was incorporated into the Workshop component on T-cell Recognition of HLA Class II Molecules. Separate Workshop components on Dw and DPw typing for identification of new specificities using homozygous typing cells (HTC) or primed lymphocyte typing (PLT) were not designed as part of the Tenth Workshop. It was, however, essential that accurate information was obtained for HLA-Dw and HLA-DPw specificities for the Reference Panel of B-Lymphoblastoid Cell Lines. A working group consisting of the authors of this paper was established for this purpose. Whenever possible, any residual PBL from the cell line donors was collected and distributed to several interested laboratories for cellular typing studies. In the case of DP, typing was also performed using the B-cell lines themselves. DP typing was performed. The results of the DP typing studies are shown in Table 1. Dw assignments were made based on data from several sources: 1) assignments in the Tenth Workshop reference laboratories in Minneapolis (US2BAC) and Seattle (US7HAN),2) assignments made in previous International or Asia-Oceania Histocompatibility Workshops, 3) analyses with cytolytic and proliferative T-cell clones, the patterns of reactivity of which were well established versus other well-typed cell lines, 4) assignments made by local laboratories, and 5) assignments made based on the Workshop analyses. The assignments, together with their source are listed in Table 2. Reprints. (kt)