Determination of Dimethyl Sulfoxide (DMSO), Ethanol (ETOH), Formamide (F) and Glycerol/Formal (GF) by High Performance Liquid Chromatography (HPLC).

摘要

Liquid chromatographic (LC) methods have been developed for the determination of dimethyl sulfoxide (DMSO), ethanol (ETOH), formamide (F), and glycerol/formal (GF), in aqueous samples. The samples are directly injected onto a high performance liquid chromatograph (HPLC) without further sample preparation. Separation of DMSO and F was achieved by using a C18 column and a mobile phase containing water/methanol. Separation of GS and ETOH was achieved by using a Dextro-Pak column and a mobile phase consisting of water. A programmable ultraviolet (UV) spectrophotometric detector was used to monitor the LC effluent for DMSO and F. A refractive index detector was used to monitor the LC effluent for ETOH and GF. The precision and accuracy of all methods are included in the report.