Interaction of Electromagnetic Fields with Biosynthetic Processes in Connective Tissue Cells.

摘要

The specific objectives of this research period were: (1) determine whether chondrocytes can be maintained in long-term (2-3 months) culture in agarose gel in order to test the effects of chronic as well as acute response to exposure to electromagnetic fields; (2) measure chondrocyte proliferation, biosynthetic rates for proteoglycans and proteins, total accumulation of proteoglycans, and physical properties of agarose/chondrocyte gel cultures; (3) construct specialized chambers for application of electric fields to agarose/chondrocyte disks in a standard incubator environment; and (4) begin experiments to measure the effects of applied current densities over a wide range of amplitudes and frequencies on biosynthesis of proteoglycans and proteins.