Cooling to Heat of Fusion (HOF), Followed by Rapid Rewarming, Does not Reduce theIntegrity of Microvascular Corrosion Casts

摘要

This study used microvascular corrosion casting techniques to evaluate changes inthe microvascular patency of rat hindpaws cooled to four different subzero temperatures. Left hindpaws of anesthetized rats in group one were cooled to -5 C, in group two to -15 C, in group three to heat of fusion (HOF), and in group four to HOF and then to -15 C. Although freezing did not take place in the hindpaws of groups one and two, initiation of freezing in the tissues, as indicated by HOF, did occur in groups three and four. Cooled hindpaws were rapidly rewarmed. Right hindpaws served as controls. Microvascular corrosion casts were made from the left and right hindpaws of all animals. There was no significant difference when the mean cast weights of cooled hindpaws from groups one, two, and three were compared to the mean cast weights of their respective control hindpaws. In group four, there was a significant difference (p<0.05) when