Simultaneous Determination of Chloroquine and Selected Calcium Channel Blockersby High-Performance Chromatography

摘要

Chloroquine (CQ), which is the primary drug for treatment and prohylaxis againstmalaria, has become ineffective because of the high prevalence of CQ-resistant P falciparum parasites, but resistant parasites exposed to a Ca (II) channel blocker become as susceptible to CQ as sensitive parasites. A reversed phase liquid chromatographic methods is described for simultaneously determining CQ, its metabolites desethyl-CQ and bisdesethyl-CQ, the Ca (II) blocker verapamil, and the tiapamil analogue N-(3,4-dimethoxyphenethyl)-N-methyl-2-(naphthyl) -m-dithiane-2-propylamine hydrochloride. The analytes were separated by gradient elution with acetonitrile and 1-heptane sulfonic acid; the detector wavelength was 232 nm. The mean recovery from spiked plasma was 100.1+ 2.28 (SD). Within-day retention times were reproducible to within +0.03 SD of mean values and the lower limit of detection was about 2 ng of each analyte. Keywords: Anti malarials, Liquid chromatography, Calcium (II) channel blockers, Malaria. (jes)